M. Lane Gilchrist

M. Lane Gilchrist

Group Website


1995-1999 NIH Postdoctoral Fellow, Department of Chemistry, Columbia University.
1989-1995 Ph.D. in Biophysical Chemistry. University of California, Davis, Department of Chemistry
1981-1987 B.S. in Chemical Engineering. Louisiana State University

Areas of Expertise

Molecular Engineering of Proteins; Biomembranes and Membrane Proteins; Fluorescence Spectroscopy and Imaging; Magnetic Resonance Spectroscopy; 

Current Research Interests

In Vitro Biomembrane Hybrid Systems
Biomembranes are amazing, multifunctional structures composed of a continuous bilayer of lipid molecules embedded with integral and peripheral membrane proteins. Membrane proteins make up ~30% of the genome of a typical eukaryotic cell, yet due largely to a strict requirement of a highly mobile, native-like biomembrane microenvironment, these molecules have not been widely built into functional materials.
Membrane protein structure is often highly complex, typified by large, multi-subunit complexes that not only span the lipid bilayer but also contain large (>2 nm) cytoplasmic and extracellular domains that protrude from the membrane. We have been researching the construction of stabilized biomembrane hybrid systems based on a biomimetic, surface-tethered artificial cytoskeleton where membrane-protein-polymer bioconjugates anchor the lipid bilayer and provide adequate biomembrane to substrate spacing. This application of this core technology is to enable in vitro supported biomembrane systems in which membrane proteins with large cytoplasmic domains can be stably interfaced with materials. Furthermore, the anchoring of biomembranes in this fashion could lead to assemblies that could hold up to shear, flow, and friction in challenging microenvironments such as found in microdevices, packed beds, and biomaterials.

Selected Publications

Zhong, L., Tu, R. S. and Gilchrist, M. L. (2013) Tether-Supported Biomembranes with alpha‐Helical Peptide-Based Anchoring Constructs Langmuir 28, 299-307

Ahn, K., Shelton, C. C., Tian, Y. M., Zhan, X, Gilchrist, M. L., Sisodia, S. S. and Li, Y-M, Intrinsic -Secretase and Presenilinase Activity of Presenilin 1. (2010) Proc Natl Acad Sci U S A 107 (50) 21435-21440

Yeagle, G.J., Gilchrist, M.L., McCarrick, R.M., Britt, R.D. Multifrequency pulsed electron paramagnetic resonance study of the S2 state of the photosystem II manganese cluster. (2008) Inorg Chem. Mar 17;47(6):1803-14

Dudu, V., Ramcharan, M., Gilchrist, M. L., Holland, E.. C., and Vazquez, M, (2008) Liposome Delivery of Quantum Dots to the Cytosol of Live Cells Journal of Nanoscience and Nanotechnology 8, 2293–2300

Vaidya, S., Gilchrist, M. L., Maldarelli, C. M., and Couzis, A, (2007) Spectral Barcoding of Polystyrene Microbeads Using Multicolored Quantum Dots, Analytical Chemistry 79, 8520–8530

Sharma, M.K. and Gilchrist, M. L., (2007) Templated Assembly of Biomembranes on Silica Micropheres using Bacteriorhodopsin Conjugates as Structural Anchors,  Langmuir 23(13):7101 - 7112

Kalyankar, N. D., Sharma, M.K., Vaidya, S., Calhoun, D.H., Maldarelli, C. M., Couzis, A. and Gilchrist, M. L., (2006) Arraying of Intact Liposomes Into Chemically Functionalized Microwell Surfaces,  Langmuir 22(12):5403-11

Vengrenyuk Y, Carlier S, Xanthos S, Cardoso L, Ganatos P, Virmani R, Einav S, Gilchrist L, Weinbaum S. A hypothesis for vulnerable plaque rupture due to stress-induced debonding around cellular microcalcifications in thin fibrous caps. (2006) Proc Natl Acad Sci U S A. Oct 3;103(40):14678-83

Sharma, M. K., Jattani, H., and Gilchrist, M. L., (2004) Bacteriorhodopsin Conjugates as Anchors for Supported Lipid Bilayers, Bioconjugate Chemistry 15(4); 942-947

Sampathkumar, P. and Gilchrist, M. L., (2004) Synthesis and Characterization of Bioconjugates of S-Layer Proteins, Bioconjugate Chemistry 15(4) 685-693

Britt, R. D., Campbell, K., Peloquin, J., Gilchrist, AM. L., Ansar, C., Dicus, M., Robbele, M., Messinger, J., (2004) Recent Pulsed EPR Studies of the Photosystem II Oxygen Evolving Complex: Implications as to Water Oxidation Mechanisms, Biochemica Et Biophysica Acta (BBA)-Bioenergetics 1655(1-3):158-71

M. Lane Gilchrist Jr., Monde, K. Tomita, Y.; Iwashita, T.; Nakanishi, K. and McDermott, A. E. (2001), Measurement of Interfluorine Distances in Solids. Journal of Magnetic Resonance, 152, 1-6

Gilchrist, M. L. Jr.; Randall, D. R.; Ball, J. A.; Britt, R. D. (1995), Proximity of the Redox Active Tyrosine Yz. to the Manganese Cluster of Photosystem II. Proceedings of the National Academy of Sciences, 92, 9545-9549

M. Lane Gilchrist
Assistant Professor of Chemical Engineering
Steinman Hall T-306
Gilchrist Lab [..]